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MyBiosource Biotechnology rabbit collagen type x elisa kit
Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by <t>ELISA</t> ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.
Rabbit Collagen Type X Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+collagen+type+x+elisa+kit/rabbit+collagen+type+x+elisa+kit/pmc09775637-208-9-15
Average 90 stars, based on 1 article reviews
rabbit collagen type x elisa kit - by Bioz Stars, 2026-10
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Article Title: The Biomimetics of Mg 2+ -Concentration-Resolved Microenvironment for Bone and Cartilage Repairing Materials Design

Journal: Biomimetics

doi: 10.3390/biomimetics7040227

Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by ELISA ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.
Figure Legend Snippet: Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by ELISA ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.

Techniques Used: Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay, ALP Activity Assay, Staining

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Enzyme-linked Immunosorbent Assay:

Article Title: The Biomimetics of Mg 2+ -Concentration-Resolved Microenvironment for Bone and Cartilage Repairing Materials Design
Article Snippet: Chemicals and materials (and their purity and manufacture) in the experiment are listed as follows: Magnesium chloride hexahydrate (99%, Aladdin, Shanghai, China), Collagenase Type II (Sigma Aldrich, St. Louis, MO, USA), Trypsin (Sigma Aldrich, St. Louis, MO, USA), Phosphate Buffer Saline (PBS, Hyclone, Cytiva, Logan, UT, USA), Penicillin–streptomycin (Hyclone, Cytiva, Logan, UT, USA), Fetal Bovine Serum (FBS, Biological Industries, Israel), Dulbecco’s modified Eagle’s Medium (DMEM) of High glucose (HG, 4500 mg/dL) (DEME-HG; Hyclone, Cytiva, Logan, UT, USA), Dulbecco’s modified Eagle’s Medium/Nutrient Mixture F12 (DEME/F12; Hyclone, Cytiva, Logan, UT, USA), Cell counting kit-8 (CCK8, Solarbio, Beijing, China), Crystal violet (Solarbio, Beijing, China), Calcein-AM/Propidium Iodide (PI) live/dead cell staining kit (Solarbio, Beijing, China), 4′,6-diamidino-2-phenylindole (DAPI, Solarbio, Beijing, China), FITC-phalloidin (Solarbio, Beijing, China), Lipopolysaccharide (LPS, Solarbio, Beijing, China), L-Ascorbic acid (Sigma Aldrich, A4403, St. Louis, MO, USA), β-Glycerophosphate disodium salt hydrate (Sigma Aldrich, G9422, St. Louis, MO, USA), Dexamethasone (Sigma Aldrich, D4902, St. Louis, MO, USA), Alkaline Phosphatase (ALP) Assay kit (Beyotime Biotechnology, Shanghai, China), BCIP/NBT Alkaline Phosphatase (ALP) Assay Chromogenic Kit (Beyotime Biotechnology, Shanghai, China), Alizarin red (Solarbio, Beijing, China), Trizol reagent (Yeason Biotech, Shanghai, China), Hifair ® II First Strand cDNA Synthesis Kit (Yeason Biotech, Shanghai, China), Hieff ® qPCR SYBR Green Master Mix (Yeason Biotech, Shanghai, China). .. Rabbit Collagen Type II ELISA Kit (Cusabio, Wuhan, China), Rabbit Collagen Type X ELISA Kit (MyBioSource, San Diego, CA, USA), Rabbit Collagen Type I ELISA Kit (Cusabio, Wuhan, China), Mouse Collagen Type I ELISA Kit (Cusabio, Wuhan, China). ..



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MyBiosource Biotechnology rabbit collagen type x elisa kit
Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by <t>ELISA</t> ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.
Rabbit Collagen Type X Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+collagen+type+x+elisa+kit/rabbit+collagen+type+x+elisa+kit/pmc09775637-208-9-15
Average 90 stars, based on 1 article reviews
rabbit collagen type x elisa kit - by Bioz Stars, 2026-10
90/100 stars
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Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by ELISA ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.

Journal: Biomimetics

Article Title: The Biomimetics of Mg 2+ -Concentration-Resolved Microenvironment for Bone and Cartilage Repairing Materials Design

doi: 10.3390/biomimetics7040227

Figure Lengend Snippet: Osteogenic-related gene expressions of MC3T3-E1 cells cultured in different concentrations of Mg 2+ ( A1 – A4 ), all data are presented as fold change after normalization to GAPDH, and fold changes are shown as mean ± SD, * p < 0.05, ** p < 0.01, and *** p < 0.001. COL I contents of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by ELISA ( B ). ALP activity of MC3T3-E1 cells after being cultured in varied Mg 2+ concentrations on the 7d by quantitative ALP activity assay ( C1 ) and ALP activity staining assay ( C2 , C3 ). Effect of Mg 2+ concentrations on the formation of calcium nodules ( D1 , D2 ). The scale bars in C2, C3, D1, and D2 are 5 mm, 200 μm, 5 mm, and 200 μm, respectively.

Article Snippet: Rabbit Collagen Type II ELISA Kit (Cusabio, Wuhan, China), Rabbit Collagen Type X ELISA Kit (MyBioSource, San Diego, CA, USA), Rabbit Collagen Type I ELISA Kit (Cusabio, Wuhan, China), Mouse Collagen Type I ELISA Kit (Cusabio, Wuhan, China).

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay, ALP Activity Assay, Staining